| 质粒名称: | pBABE BRG1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 1959 |
| 存储实验室: | Robert Kingston |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-puro |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5200 bp |
| 完整质粒大小: | 10200 bp |
| 载体类型: | 哺乳细胞表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BRG1 |
| 物种来源: | 人源 |
| 插入大小: | 5000 bp |
| 基因又名: | BAF190a |
| 标签/融合蛋白: | flag (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SalI(未破坏) |
| 3' 克隆位点: | SalI (未破坏) |
| 5' 测序引物 | pBABE 5' |
|
文献引用方法 |
|
| 材料和方法部分: | pBABE BRG1 was a gift from Robert Kingston (爱迪基因 plasmid # 1959) |
| 参考文献部分: | Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380 |
pBABE BRG1
价格：1000元
联系方式：I47-825O-882O
买家导航
pBABEBRG1质粒基本信息
pBABEBRG1质粒图谱信息
pBABEBRG1质粒简介
pBABEBRG1质粒序列
LOCUS pBABE BRG1 10182 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABE BRG1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10182)
AUTHORS Sif S, Saurin AJ, Imbalzano AN, Kingston RE
TITLE Purification and characterization of mSin3A-containing Brg1 and hBrm
chromatin remodeling complexes.
JOURNAL Genes Dev 2001 Mar 1;15(5):603-18.
PUBMED 11238380
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pBABE BRG1|
FEATURES Location/Qualifiers
source 1..10182
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 59..155
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 5096..5119
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(5137..5157)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5142..5471
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5322..5457
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5384..5403
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5481..6080
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5481..5500)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5977..5997
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6301..6770
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6900..6917)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7071..7659)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7151..7170)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7819..8679)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8442..8461
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8680..8784)
/gene="bla"
/label=AmpR promoter
LTR 8815..9284
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 9349..9548
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 9749..10165
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 10095..10117
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 10133..10149
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
ORIGIN
1 gtggtacgta ggaattcgcc agcacagtgg tcgacggtat cgataagctt gataactggt
61 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag
121 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
181 tctaaaagct gctctagccc tcgacggcgc cggcggctcc tgcaggaggc cactgtctgc
241 agctcccgtg aagatgtcca ctccagaccc acccctgggc ggaactcctc ggccaggtcc
301 ttccccgggc cctggccctt cccctggagc catgctgggc cctagcccgg gtccctcgcc
361 gggctccgcc cacagcatga tggggcccag cccagggccg ccctcagcag gacaccccat
421 ccccacccag gggcctggag ggtaccctca ggacaacatg caccagatgc acaagcccat
481 ggagtccatg catgagaagg gcatgtcgga cgacccgcgc tacaaccaga tgaaaggaat
541 ggggatgcgg tcagggggcc atgctgggat ggggcccccg cccagcccca tggaccagca
601 ctcccaaggt tacccctcgc ccctgggtgg ctctgagcat gcctctagtc cagttccagc
661 cagtggcccg tcttcggggc cccagatgtc ttccgggcca ggaggtgccc cgctggatgg
721 tgctgacccc caggccttgg ggcagcagaa ccggggccca accccattta accagaacca
781 gctgcaccag ctcagagctc agatcatggc ctacaagatg ctggccaggg ggcagcccct
841 ccccgaccac ctgcagatgg cggtgcaggg caagcggccg atgcccggga tgcagcagca
901 gatgccaacg ctacctccac cctcggtgtc cgcaacagga cccggccctg gccctggccc
961 tggccccggc ccgggtcccg gcccggcacc tccaaattac agcaggcctc atggtatggg
1021 agggcccaac atgcctcccc caggaccctc gggcgtgccc cccgggatgc caggccagcc
1081 tcctggaggg cctcccaagc cctggcctga aggacccatg gcgaatgctg ctgcccccac
1141 gagcacccct cagaagctga ttcccccgca gccaacgggc cgcccttccc ccgcgccccc
1201 tgccgtccca cccgccgcct cgcccgtgat gccaccgcag acccagtccc ccgggcagcc
1261 ggcccagccc gcgcccatgg tgccactgca ccagaagcag agccgcatca cccccatcca
1321 gaagccgcgg ggcctcgacc ctgtggagat cctgcaggag cgcgagtaca ggctgcaggc
1381 tcgcatcgca caccgaattc aggaacttga aaaccttccc gggtccctgg ccggggattt
1441 gcgaaccaaa gcgaccattg agctcaaggc cctcaggctg ctgaacttcc agaggcagct
1501 gcgccaggag gtggtggtgt gcatgcggag ggacacagcg ctggagacag ccctcaatgc
1561 taaggcctac aagcgcagca agcgccagtc cctgcgcgag gcccgcatca ctgagaagct
1621 ggagaagcag cagaagatcg agcaggagcg caagcgccgg cagaagcacc aggaatacct
1681 caatagcatt ctccagcatg ccaaggattt caaggaatat cacagatccg tcacaggcaa
1741 aatccagaag ctgaccaagg cagtggccac gtaccatgcc aacacggagc gggagcagaa
1801 gaaagagaac gagcggatcg agaaggagcg catgcggagg ctcatggctg aagatgagga
1861 ggggtaccgc aagctcatcg accagaagaa ggacaagcgc ctggcctacc tcttgcagca
1921 gacagacgag tacgtggcta acctcacgga gctggtgcgg cagcacaagg ctgcccaggt
1981 cgccaaggag aaaaagaaga aaaagaaaaa gaagaaggca gaaaatgcag aaggacagac
2041 gcctgccatt gggccggatg gcgagcctct ggacgagacc agccagatga gcgacctccc
2101 ggtgaaggtg atccacgtgg agagtgggaa gatcctcaca ggcacagatg cccccaaagc
2161 cgggcagctg gaggcctggc tcgagatgaa cccggggtat gaagtagctc cgaggtctga
2221 tagtgaagaa agtggctcag aagaagagga agaggaggag gaggaagagc agccgcaggc
2281 agcacagcct cccaccctgc ccgtggagga gaagaagaag attccagatc cagacagcga
2341 tgacgtctct gaggtggacg cgcggcacat cattgagaat gccaagcaag atgtcgatga
2401 tgaatatggc gtgtcccagg cccttgcacg tggcctgcag tcctactatg ccgtggccca
2461 tgctgtcact gagagagtgg acaagcagtc agcgcttatg gtcaatggtg tcctcaaaca
2521 gtaccagatc aaaggtttgg agtggctggt gtccctgtac aacaacaacc tgaacggcat
2581 cctggccgac gagatgggcc tggggaagac catccagacc atcgcgctca tcacgtacct
2641 catggagcac aaacgcatca atgggccctt cctcatcatc gtgcctctct caacgctgtc
2701 caactgggcg tacgagtttg acaagtgggc cccctccgtg gtgaaggtgt cttacaaggg
2761 atccccagca gcaagacggg cctttgtccc ccagctccgg agtgggaagt tcaacgtctt
2821 gctgacgacg tacgagtaca tcatcaaaga caagcacatc ctcgccaaga tccgttggaa
2881 gtacatgatt gtggacgaag gtcaccgcat gaagaaccac cactgcaagc tgacgcaggt
2941 gctcaacacg cactatgtgg caccccgccg cctgctgctg acgggcacac cgctgcagaa
3001 caagcttccc gagctctggg cgctgctcaa cttcctgctg cccaccatct tcaagagctg
3061 cagcaccttc gagcagtggt ttaacgcacc ctttgccatg accggggaaa aggtggacct
3121 gaatgaggag gaaaccattc tcatcatccg gcgtctccac aaagtgctgc ggcccttctt
3181 gctccgacga ctcaagaagg aagtcgaggc ccagttgccc gaaaaggtgg agtacgtcat
3241 caagtgcgac atgtctgcgc tgcagcgagt gctctaccgc cacatgcagg ccaagggcgt
3301 gctgctgact gatggctccg agaaggacaa gaagggcaaa ggcggcacca agaccctgat
3361 gaacaccatc atgcagctgc ggaagatctg caaccacccc tacatgttcc agcacatcga
3421 ggagtccttt tccgagcact tggggttcac tggcggcatt gtccaagggc tggacctgta
3481 ccgagcctcg ggtaaatttg agcttcttga tagaattctt cccaaactcc gagcaaccaa
3541 ccacaaagtg ctgctgttct gccaaatgac ctccctcatg accatcatgg aagattactt
3601 tgcgtatcgc ggctttaaat acctcaggct tgatggaacc acgaaggcgg aggaccgggg
3661 catgctgctg aaaaccttca acgagcccgg ctctgagtac ttcatcttcc tgctcagcac
3721 ccgggctggg gggctcggcc tgaacctcca gtcggcagac actgtgatca tttttgacag
3781 cgactggaat cctcaccagg acctgcaagc gcaggaccga gcccaccgca tcgggcagca
3841 gaacgaggtg cgtgtgctcc gcctctgcac cgtcaacagc gtggaggaga agatcctagc
3901 tgcagccaag tacaagctca acgtggacca gaaggtgatc caggccggca tgttcgacca
3961 gaagtcctcc agccatgagc ggcgcgcctt cctgcaggcc atcctggagc acgaggagca
4021 ggatgaggag gaagacaagg tgcccgacga cgagaccgtc aaccagatga tcgcccggca
4081 cgaggaggag tttgatctgt tcatgcgcat ggacctggac cgcaggcgcg aggaggcccg
4141 caaccccaag cggaagccgc gcctcatgga ggaggacgag ctcccctcgt ggatcatcaa
4201 ggacgacgcg gaggtggagc ggctgacctg tgaggaggag gaggagaaga tgttcggccg
4261 tggctcccgc caccgcaagg aggtggacta cagcgactca ctgacggaga agcagtggct
4321 caaggccatc gaggagggca cgctggagga gatcgaagag gaggtccggc agaagaaatc
4381 atcacggaag cgcaagcgag acagcgacgc cggctcctcc accccgacca ccagcacccg
4441 cagccgcgac aaggacgacg agagcaagaa gcagaagaag cgcgggcggc cgcctgccga
4501 gaaactctcc cctaacccac ccaacctcac caagaagatg aagaagattg tggatgccgt
4561 gatcaagtac aaggacagca gcagtggacg tcagctcagc gaggtcttca tccagctgcc
4621 ctcgcgaaag gagctgcccg agtactacga gctcatccgc aagcccgtgg acttcaagaa
4681 gataaaggag cgcattcgca accacaagta ccgcagcctc aacgacctag agaaggacgt
4741 catgctcctg tgccagaacg cacagacctt caacctggag ggctccctga tctatgaaga
4801 ctccatcgtc ttgcagtcgg tcttcaccag cgtgcggcag aaaatcgaga aggaggatga
4861 cagtgaaggc gaggagagtg aggaggagga agagggcgag gaggaaggct ccgaatccga
4921 atctcggtcc gtcaaagtga agatcaagct tggccggaag gagaaggcac aggaccagct
4981 gaagggcggc cggcggcggc cgagccgagg gtcccgagcc aagccggtcg tgagtgacga
5041 tgacagtgag gaggaacaag aggaggaccg ctcaggaagt ggcagcgaag aagacgacta
5101 caaggacgac gatgacaaat gataagtcga ccctgtggaa tgtgtgtcag ttagggtgtg
5161 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
5221 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
5281 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
5341 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
5401 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
5461 gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
5521 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
5581 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
5641 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
5701 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
5761 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
5821 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
5881 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
5941 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
6001 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
6061 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
6121 cacgacccca tgcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
6181 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
6241 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
6301 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
6361 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
6421 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
6481 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
6541 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
6601 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
6661 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
6721 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
6781 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat
6841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
6901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
6961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
7021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
7081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
7141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
7201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
7261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
7321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
7381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
7441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
7501 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
7741 tcaaaaagga tcttcaccta gatccttttg cggccgcaaa tcaatctaaa gtatatatga
7801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
7861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
7921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
7981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
8041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
8101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
8161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
8221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
8281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
8341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
8401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
8461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
8521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
8581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
8641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
8701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
8761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cagcctgaat
8821 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
8881 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
8941 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
9001 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
9061 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
9121 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
9181 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
9241 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
9301 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa gctggccagc
9361 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
9421 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
9481 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
9541 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
9601 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
9661 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
9721 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
9781 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
9841 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
9901 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
9961 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
10021 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
10081 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
10141 agccctcact ccttctctag gcgccggccg gatcccagtg tg
//
